Renal cell carcinoma (RCC) accounts for a substantial proportion of cancer cases worldwide, with particularly high incidence rates observed in Asian populations. According to existing studies on RCC and super enhancers (SEs), the kinase PIM3 (Pim-3 proto-oncogene, serine/threonine kinase) is found to be overexpressed in RCC. Since kinase proteins are generally considered feasible targets for drug development, this study focused on PIM3 as the primary subject of investigation. Through statistical analyses of tumor samples from clinical RCC patients and the establishment of PIM3 knock-down cell lines using gene-editing techniques, we observed significant changes in RCC cell growth in various aspect, demonstrating a strong correlation between PIM3 expression and RCC proliferation. These findings not only further establish the link between SEs and cancer progression but also enhance our understanding of the pathogenic mechanisms and key genes involved in RCC, providing valuable insights for the development of novel targeted therapies for this disease.