In the Nordic region, residents are prone to Seasonal Affective Disorder (SAD), and the cold climate often limits crop growth, leading to food shortages that can heighten hunger and psychological anxiety. Given existing data suggesting sex differences in coping with stress, this study aimed to investigate the relationship between circadian rhythm disruption, hunger, and anxiety levels, and to analyze whether there are sex differences in stress adaptability. 　　　　This research utilized zebrafish (Danio rerio) as animal model, focusing on the relationship among anxiety, darkness, and hunger, and assessing how sex differences influence stress adaptation. Behavioral changes were observed using the Novel Tank test and the Cued Fear test, applying different durations of environmental manipulation to evaluate their effects on anxiety levels. Additionally, brain staining experiments were conducted to quantify activated nerve cells, using phospho-S6 Ribosomal protein (pS6) as a marker in the griseum centrale (GC) brain region, to provide further insights into anxiety-related neural activity. The study also evaluated the dose-dependent effects of the anxiolytic compound RS102221 on zebrafish anxiety levels. 　　　　Results indicated that both darkness and fasting increased anxiety levels in zebrafish. Fasting caused a greater elevation in anxiety and reduced activity compared to lack of light alone. Increasing the duration of darkness was positively correlated with increased anxiety. While behavioral tests showed mixed results regarding sex differences, the brain staining experiment suggested that male zebrafish were more sensitive to the stimulus and stress (darkness/fasting combined) compared to females, exhibiting a higher number of activated nerve cells in the GC region. Furthermore, the anxiolytic compound RS102221 demonstrated dose-dependent effects; the 3μM concentration effectively reduced anxiety, whereas the 6μM concentration did not show a similar relieving effect. These findings highlight the significant impact of environmental factors and routine on emotional stability, providing potential applications for understanding human psychological health and stress management strategies across genders.