This study investigated the carbon sequestration capacity of campus trees using two complementary approaches: growth measurement methods and photosynthesis analysis. We quantified carbon storage for all tree species on campus and examined seasonal variations in carbon absorption efficiency for two representative species, Melia azedarach (chinaberry) and Diospyros discolor (velvet apple). Results showed that 927 trees on campus sequestered 197.36 tons of carbon, equivalent to 723.65 tons of CO₂. Carbon sequestration was concentrated in four dominant species—banyan trees, Casuarina equisetifolia, Pterocarpus indicus, and rubber trees—which collectively accounted for 61% of total campus carbon storage. These findings suggest that future campus forestation strategies should incorporate both fast-growing and long-lived tree species to optimize both immediate and sustained carbon sequestration benefits. Photosynthesis experiments revealed distinct seasonal patterns and species-specific characteristics in carbon absorption. Both M. azedarach and D. discolor achieved maximum carbon sequestration during summer months. M. azedarach demonstrated higher total annual carbon absorption compared to D. discolor, but exhibited greater seasonal variability in performance. Conversely, D. discolor maintained more stable, albeit lower, carbon sequestration rates throughout the year and showed superior carbon absorption efficiency per unit leaf area. Finally, we conducted a comprehensive carbon footprint analysis by calculating electricity-related carbon emissions across campus and evaluating the potential for carbon neutrality. This holistic assessment provides valuable insights for developing effective campus sustainability strategies and understanding the role of urban forestry in mitigating institutional carbon emissions. This research demonstrates the significant contribution of campus trees to carbon sequestration and highlights the importance of strategic species selection in maximizing long-term environmental benefits.